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Living Modified Organism
(LMO)
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Insect-resistant rice
EN
KMD1 (Kemingdao1)
No
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Organization:Zhejiang University (ZJU)Academic or research institute866 Yuhangtang RdHangzhou, Zhejiang
310058, ChinaPhone: +86 571 8820 6236, +86 571 8898 2268, +86 571 8820 6480,Fax:Website: https://www.zju.edu.cn/,
The rice (Oryza sativa) was modified for insect resistance through the expression of Bacillus thuringiensis Cry1Ab (Bt) protein, which disrupts the insect's midgut through pore formation. In addition to the Bt protein, the rice expresses, Escherichia coli hygromycin B phosphotransferase and neomycin phosphotransferase II, for hygromycin and kanamycin resistance and E. coli beta-glucuronidase, as a reporter gene. These are/were likely used as selectable markers.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-10454-5 Organism Oryza sativa (Rice, ORYSA)Crops
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pKUB
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100292-5 Hygromycin B phosphotransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to antibiotics (Hygromycin)
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
The modified rice contains four gene cassettes: Escherichia coli neomycin phosphotransferase II (nptII); Bacillus thuringiensis cry1Ab; E. coli hygromycin B phosphotransferase (hpt); and E. coli beta-glucuronidase (uidA).
The nptII sequence is under control of an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.
The cry1Ab sequence is under control of a Zea mays ubiquitin promoter and nos terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected to occur in all plant tissues.
The hpt coding sequence is under control of a Cauliflower mosaic virus 35S promoter and nos terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected to occur in all plant tissues.
The uidA coding sequence is under control of a Cauliflower mosaic virus 35S promoter and nos terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected to occur in all plant tissues.
The nptII sequence is under control of an Agrobacterium tumefaciens nopaline synthase (nos) promoter and terminator.
The cry1Ab sequence is under control of a Zea mays ubiquitin promoter and nos terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected to occur in all plant tissues.
The hpt coding sequence is under control of a Cauliflower mosaic virus 35S promoter and nos terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected to occur in all plant tissues.
The uidA coding sequence is under control of a Cauliflower mosaic virus 35S promoter and nos terminator. Due to the constitutive nature of the promoter, high levels of transcription are expected to occur in all plant tissues.
EN
- Food
- Research
EN
EN
